Improved technique for staining bacteria on membrane filters
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چکیده
منابع مشابه
Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.
Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black Uni-Pore membrane filt...
متن کاملA technique for detecting Treponema pallidum through the use of membrane filters and immunofluorescence staining.
Danielsson (1965) has recently described the use of non-fluorescent membrane filters and immunofluorescence staining for the identification of certain bacteria in water. The principal advantages of the procedure were that it was rapid; that it could separate and identify a small number of organisms from a relatively large volume of fluid; and that it allowed a quantitative estimate of the conce...
متن کاملSelective staining of cytoplasmic membrane and nuclear apparatus of bacteria.
A selective and simultaneous staining method for nuclear apparatus and cytoplasmic membrane of some bacteria has been presented. Nuclear apparatus is stained with basic fuchsin after hydrolysis with I N HCl and cytoplasmic membrane is restained with Victoria blue 4R after treating with saturated mercuric chloride. By this method, the nuclear apparatuses of B. subtilis, Sal. typhi 57 and Staph. ...
متن کاملGrowth of silicone-immobilized bacteria on polycarbonate membrane filters, a technique to study microcolony formation under anaerobic conditions.
A technique was developed to study microcolony formation by silicone-immobilized bacteria on polycarbonate membrane filters under anaerobic conditions. A sudden shift to anaerobiosis was obtained by submerging the filters in medium which was depleted for oxygen by a pure culture of bacteria. The technique was used to demonstrate that preinduction of nitrate reductase under low-oxygen conditions...
متن کاملImproved brilliant blue G staining of the internal limiting membrane with sharp cut filters of a novel viewing filter system.
PURPOSE We developed a new artificial image enhancement system aimed at intraoperative visibility improvement as a clinical prototype. We examined each optical characteristic and change in intraoperative visibility using brilliant blue G (BBG) staining with various sharp cut filters (SCFs). METHOD This was a retrospective and observational study. The system was composed of several filters att...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1968
ISSN: 0003-6919
DOI: 10.1128/am.16.2.433-434.1968